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Effects of 3,4-diaminopyridine on myoplasmic calcium and phosphoinositide hydrolysis in frog sartorius muscle fibers

   
@article{APS6182,
	author = {Ze-min Xu and Zheng Dong and Pei-hong Zhu},
	title = {Effects of 3,4-diaminopyridine on myoplasmic calcium and phosphoinositide hydrolysis in frog sartorius muscle fibers},
	journal = {Acta Pharmacologica Sinica},
	volume = {15},
	number = {2},
	year = {2016},
	keywords = {},
	abstract = {Using Ca(2+)-selective microelectrodes, the effects of 3,4-diaminopyridine (DAP) on myoplasmic calcium ([Ca2+]i) were studied in frog satorius muscle fibers. DAP induced an increase of [Ca2+]i in a concentration-dependent manner. DAP 1 mmol.L-1 increased the [Ca2+]i from control 166 +/- 41 nmol.L-1 to 416 +/- 69 nmol.L-1 (n = 10). In the absence of external calcium, DAP still enhanced [Ca2+]i. [Ca2+]i of the fibers bathed in Ca(2+)-free Ringer's solution containing DAP 1 mmol.L-1 was 152 +/- 43 nmol.L-1, which was significantly higher than 77 +/- 35 nmol.L-1 of [Ca2+]i of the fibers in Ca(2+)-free Ringer's solution. In addition, DAP promoted the hydrolysis of phosphoinositides, and DAP-induced hydrolysis was more in the presence of external calcium. It is suggested that, through enhancing the hydrolysis of phosphoinositides, DAP released Ca2+ from intracellular Ca2+ store in frog sartorius muscle fibers},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/6182}
}