@article{APS6117,
author = {Ammar Alibrahim and Li-yan Zhao and Christine You-jin Bae and Andrew Barszczyk and Christopher Lf Sun and Guan-lei Wang and Hong-shuo Sun},
title = {Neuroprotective effects of volume-regulated anion channel blocker DCPIB on neonatal hypoxic-ischemic injury},
journal = {Acta Pharmacologica Sinica},
volume = {34},
number = {1},
year = {2016},
keywords = {},
abstract = {Ammar ALIBRAHIM1, 2, Li-yan ZHAO5, Christine You-jin BAE2, Andrew BARSZCZYK2, Christopher LF SUN2, Guan-lei WANG5, *, Hong-shuo SUN1, 2, 3, 4, *
1Departments of Surgery, 2Physiology and 3Pharmacology, 4Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada M5S 1A8; 5Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
Aim: To evaluate the role of swelling-induced activation of volume-regulated anion channels (VRACs) in a neonatal hypoxic-ischemic injury model using the selective VRAC blocker 4-(2-butyl-6,7-dichloro-2-cyclopentyl-indan-1-on5-yl) oxobutyric acid (DCPIB).
Methods: Cerebral hypoxic-ischemic injury was induced in 7-day-old mouse pups with Rice-Vannucci method. Prior to the onset of ischemia, the animals were ip administered DCPIB (10 mg/kg). The animals were sacrificed 24 h afterwards, coronal sections of the brains were cut and the areas of infarct were examined using TTC staining and an image-analysis system. Cultured PC12 cells were subjected to oxygen-glucose deprivation (OGD) for 4 h. The cellular viability was assessed using Cell Counting Kit 8. Intracellular chloride concentration [Cl–]i was measured using 6-methoxy-N-ethylquinolinium iodide.
Results: DCPIB-treated mice showed a significant reduction in hemispheric corrected infarct volume (26.65%±2.23%) compared to that in vehicle-treated mice (45.52%±1.45%, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6117}
}