@article{APS6047,
author = {Young-whan Choi and Kyeok Kim and Ji-yeong Jo and Hyo-lim Kim and You-jin Lee and Woo-jung Shin and Santosh J Sacket and Mijin Han and Dong-soon Im},
title = {Wuweizisu C from Schisandra chinensis decreases membrane potential in C6 glioma cells},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {9},
year = {2016},
keywords = {},
abstract = {Aim: To study the effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis, such as wuweizisu C, gomisin N, gomisin A, and schisandrin, on the membrane potential in C6 glioma cells.
Methods: The membrane potential was estimated by measuring the fluorescence change in DiBAC-loaded glioma cells.
Results: Wuweizisu C decreased the membrane potential in a concentration-dependent manner. Gomisin N and gomisin A, however, showed differential modulation and no change was induced by schisandrin or dimethyl-4,4′-dimethoxy-5,6,5′,6′-dimethylene dioxybipheny 1-2,2′-dicarboxylate, a synthetic drug derived from dibenzocyclooctadiene lignans. We found no involvement of Gi/o proteins, phospholipase C, and extracellular Na+ on the wuweizisu C-induced decrease of the membrane potential. Wuweizisu C by itself did not change the intracellular Ca2+ [Ca2+]i concentration, but decreased the ATP-indu-ced Ca2+ increase in C6 glioma cells. The 4 lignans at all concentrations used in this study did not induce any effect on cell viability. Furthermore, we found a similar decrease of the membrane potential by wuweizisu C in PC12 neuronal cells.
Conclusion: Our results suggest that the decrease in the membrane potential and the modulation of [Ca2+]i concentration by wuweizisu C could be important action mechanisms of wuweizisu C.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6047}
}