@article{APS5964,
author = {Cheng-biao Lu and Zhi-hua Wang and Yan-hong Zhou and Martin Vreugdenhil},
title = {Temperature- and concentration-dependence of kainate-induced γ oscillation in rat hippocampal slices under submerged condition},
journal = {Acta Pharmacologica Sinica},
volume = {33},
number = {2},
year = {2016},
keywords = {},
abstract = {Aim: Fast neuronal network oscillation at the γ frequency band (γ oscillation: 30–80 Hz) has been studied extensively in hippocampal slices under interface recording condition. The aim of this study is to establish a method for recording γ oscillation in submerged hippocampal slices that allows simultaneously monitoring γ oscillation and the oscillation-related intracellular events, such as intracellular Ca2+ concentration or mitochondrial membrane potentials.
Methods: Horizontal hippocampal slices (thickness: 300 μm) of adult rats were prepared and placed in a submerged or an interface chamber. Extracellular field recordings were made in the CA3c pyramidal layer of the slices. Kainate, an AMPA/kainate receptor agonist, was applied via perfusion. Data analysis was performed off-line.
Results: Addition of kainate (25–1000 nmol/L) induced γ oscillation in both the submerged and interface slices. Kainate increased the γ power in a concentration-dependent manner, but the duration of steady state oscillation was reduced at higher concentrations of kainate. Long-lasting γ oscillation was maintained at the concentrations of 100–300 nmol/L. Under submerged condition, γ oscillation was temperature-dependent, with the maximum power achieved at 29 °C. The induction of γ oscillation under submerged condition also required a fast rate of perfusion (5–7 mL/min) and showed a fast dynamic during development and after the washout.
Conclusion: The kainite-induced γ oscillation recorded in submerged rat hippocampal slices is useful for studying the intracellular events related to neuronal network activities and may represent a model to reveal the mechanisms underlying the normal neuronal synchronizations and diseased conditions.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/5964}
}