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Enhancement of antitumor activity of bleomycin A5 in mouse sarcoma 180 cells in vitro and in vivo by verapamil

  
@article{APS5695,
	author = {Nong-gao HE and Hong-qing ZHANG and Rui-hong WANG and Xin-lin YANG and Shao-bai XUE},
	title = {Enhancement of antitumor activity of bleomycin A5 in mouse sarcoma 180 cells in vitro and in vivo by verapamil},
	journal = {Acta Pharmacologica Sinica},
	volume = {11},
	number = {4},
	year = {2016},
	keywords = {},
	abstract = {Verapamil (Ver), a calcium influx blocker, enhanced the cytotoxicity of bleomycin A5 (BLM) in cultured S-180 cells. IC50 of BLM to the cells was about 2.6 micrograms/ml when the cells were treated with BLM alone, but when the cells were treated with BLM plus Ver 1, 5, 10 micrograms/ml, the IC50 were 1/1.2, 1/5.2, 1/7.4 of the cells treated with BLM alone, respectively. In colony test, the survival fractions of the cells treated with BLM 20, 60, 100 micrograms/ml plus Ver 60 micrograms/ml were 1/7, 1/8, 1/11 of the cells treated with BLM alone, respectively. Ver enhanced the growth inhibitory actions of BLM in mice bearing S-180 sarcoma by 60% or over. The mean survival the growth inhibitory actions of BLM in mice bearing S-180 sarcoma by 60% or over. The mean survival time of the mice bearing S-180 ascites sarcoma treated with BLM plus Ver was prolonged 21% from that of the mice treated with BLM alone. The results suggest that Ver may be used clinically as an antitumor enhancer of BLM.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/5695}
}