@article{APS5545,
author = {Sunny Yang Xiang and Linda L Ye and Li-lu Marie Duan and Li-hui Liu and Zhi-dong Ge and John A Auchampach and Garrett J Gross and Dayue Darrel Duan},
title = {Characterization of a critical role for CFTR chloride channels in cardioprotection against ischemia/reperfusion injury},
journal = {Acta Pharmacologica Sinica},
volume = {32},
number = {6},
year = {2016},
keywords = {},
abstract = {Aim: To further characterize the functional role of cystic fibrosis transmembrane conductance regulator (CFTR) in early and late (second window) ischemic preconditioning (IPC)- and postconditioning (POC)-mediated cardioprotection against ischemia/reperfusion (I/R) injury.
Methods: CFTR knockout (CFTR−/−) mice and age- and gender-matched wild-type (CFTR+/+) and heterozygous (CFTR+/-) mice were used. In in vivo studies, the animals were subjected to a 30-min coronary occlusion followed by a 40-min reperfusion. In ex vivo (isolate heart) studies, a 45-min global ischemia was applied. To evaluate apoptosis, the level of activated caspase 3 and TdT-mediated dUTP-X nick end labeling (TUNEL) were examined.
Results: In the in vivo I/R models, early IPC significantly reduced the myocardial infarct size in wild-type (CFTR+/+) (from 40.4%±5.3% to 10.4% ±2.0%, n=8, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/5545}
}