@article{APS5518,
author = {Yong Wang and Yan Chen and Zi Chen and Qing Wu and Wen-juan Ke and Qiu-ling Wu},
title = {Gambogic acid induces death inducer-obliterator 1-mediated apoptosis in Jurkat T cells},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To explore the anticancer effects and the molecular mechanisms of gambogic acid (GA) on Jurkat cells.
Methods: Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Annexin V-fluorescein-isothiocyanate/propidium iodide, DNA defragmentation, and comet assay were used to detect apoptosis. Western blotting was used to study the expression of death inducer-obliterator-1 (DIO-1), Bcl-2, NF-κB, and procaspase 3, as well as 2 activated subunits: p17 and p20. The subcellular localization of DIO-1 was examined by immunofluorescence and Hoechst33258 staining.
Results: GA inhibited the proliferation of Jurkat cells with 50% inhibitory concentration values of 1.51±0.09 (24 h), 0.98±0.13 (48 h), and 0.67±0.12 μmol/L (72 h). GA was able to induce apoptosis of Jurkat cells. Treated by GA, the expression of DIO-1 was upregulated, and that of Bcl-2 and NF-κB was downregulated, leading to the activation of pro-caspase 3. GA induced the translocation of DIO-1 to the nucleus.
Conclusion: GA suppressed the proliferation of Jurkat cells by apoptosis induction. DIO-1 triggered early-stage cell death in GA-treated Jurkat cells.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/5518}
}