@article{APS5517,
author = {Cheol Park and Dong-oh Moon and Chung-ho Ryu and Byung tae Choi and Won ho Lee and Gi-young Kim and Yung hyun Choi},
title = {β-Sitosterol sensitizes MDA-MB-231 cells to TRAIL-induced apoptosis},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To investigate whether subtoxic concentration of β-sitosterol (SITO) combined with TNF-related apoptosis-inducing ligand (TRAIL) induces apoptosis in TRAIL-resistant MDA-MB-231 breast cancer cells.
Methods: Cell viability and growth were assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphnyl-2H-tetrazolim bromide assays, chromatin condensation, release of lactate dehydrogenase (LDH), and Annexin V+cells. The apoptosis-related proteins were detected by Western blotting.
Results: Treatment with TRAIL in combination with subtoxic concentrations of SITO sensitized MDA-MB-231 breast cancer cells to TRAIL-mediated apoptosis. The synergistic treatment induced chromatin condensation, DNA fragmentation, the release of LDH, and Annexin V+cells. The indicators of apoptosis are correlated to the induction of caspase activities, which results in the cleavage of poly(ADP-ribose)polymerase. Both the cytotoxic effects and apoptotic characteristics induced by the synergistic treatment were significantly inhibited by a pan-caspase inhibitor z-VAD-fmk, demonstrating the important role of caspases. These results indicate that caspases are crucial regulators of apoptosis induced by the combined treatment of SITO and TRAIL in MDA-MB-231 cells.
Conclusion: The synergistic treatment of SITO and TRAIL induces apoptosis, which can serve as a potential preventive and therapeutic agent.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/5517}
}