@article{APS5512,
author = {Chang-ye Zou and Jin Wang and Jing-nan Shen and Gang Huang and Song Jin and Jun-qiang Yin and Qian-chen Guo and Hao-miao Li and Lan Luo and Meng Zhang and Long-juan Zhang},
title = {Establishment and characteristics of two syngeneic human osteosarcoma cell lines from primary tumor and skip metastases},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To characterize and compare the different biological behaviors of 2 novel human osteosarcoma cell lines, Zos and Zos-M, established respectively from the primary tumor and the skip metastasis of an osteosarcoma patient.
Methods: In vitro studies included morphological observations, karyotype analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation assay, and cell sensitivity to chemotherapeutic drugs. Subcutaneous and intravenous inoculations into nude mice were carried out to study the tumorigenicity and the metastatic potential. RT-PCR was performed to assess the expression of the osteoblastic markers and some metastasis-related genes.
Results: Both cell lines remained stable for more than 100 passages in vitro without interruption. The RT-PCR examination indicated that they retained the molecular characteristics of an osteoblastic lineage. The karyotype analysis displayed aneuploidy and various structural abnormalities. Both cell lines are tumorigenic; Zos-M differs from Zos by the former's ability to develop lung metastasis after intravenous injection. The comparison of the expression patterns of some metastasis-related genes revealed that the decreased expression of cadherin-11 in Zos-M may correlate with a high potential of metastases. Moreover, both cell lines are less sensitive to the current chemotherapy protocols.
Conclusion: The establishment of osteosarcoma cell lines, Zos and Zos-M, and related animal models provide a useful resource for studying the aggressive behavior of osteosarcoma and will be helpful for screening effective treatment strategies.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/5512}
}