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Myosin light chain kinase is responsible for high proliferative ability of breast cancer cells via anti-apoptosis involving p38 pathway

   
@article{APS5398,
	author = {Wen-jing Cui and Yi Liu and Xiao-lei Zhou and Feng-ze Wang and Xiao-dong Zhang and Li-hong Ye},
	title = {Myosin light chain kinase is responsible for high proliferative ability of breast cancer cells via anti-apoptosis involving p38 pathway},
	journal = {Acta Pharmacologica Sinica},
	volume = {31},
	number = {6},
	year = {2016},
	keywords = {},
	abstract = {Aim: To investigate whether myosin light chain kinase (MLCK) contributed to the high proliferative ability of breast cancer cells.
 Methods: Soft agar colony formation on the MCF-7 and LM-MCF-7 cell lines was determined. The cell cycles of MCF-7 and LM-MCF-7 were detected using flow cytometry analysis. Western blot analysis was performed to detect the expression levels of p-ERK1/2, total-ERK1/2, p-p38, total p38, p-JNK, total-JNK, survivin, Bcl-2, p-MLC, caspase-9, cleaved caspase-9, and MLCK. After treatment with adriamycin (ADR), ML-7 and SB203580, apoptosis was examined using flow cytometry analysis and Annexin V-FITC fluorescence microscopy.
 Results: The breast cancer LM-MCF-7 cell line with high metastasis potential (a metastitic sub-clone of MCF-7) had higher anti-apoptosis ability relative to MCF-7 cells in response to adriamycin treatment (apoptosis rate: 6.76% vs 28.58%, P},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/5398}
}