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Isolation and purification of active opioid receptors from rat brain

   
@article{APS5239,
	author = {Feng Wang and Zhi-Qiang Chi},
	title = {Isolation and purification of active opioid receptors from rat brain},
	journal = {Acta Pharmacologica Sinica},
	volume = {8},
	number = {6},
	year = {2016},
	keywords = {},
	abstract = {Active opioid receptor was purified to apparent homogeneity from rat brain membranes. The purification was accomplished in two steps: Vicia bungei Ohwi lectin-sepharose affinity chromatography followed by 6-succinylmorphine-(CH2)6-sepharose affinity chromatography. The purified receptor had a molecular weight of 45 000 as determined by SDS-PAGE and was judged to be homogeneous and genuine opioid receptor by the following criteria： (1) and (2) the Na125I andβ-[125I]endorphin labeled protein was analysed by SDS-PAGE separately and a single band was detected in both； (3) a single band was shown in the gel of isoelectric focusing and (4) the purified protein bound with [3H]etorphine in a stereospecific and saturable manner.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/5239}
}