@article{APS4900,
author = {Qi Zhang and Gang Bai and Jia-qi Chen and Wang Tian and Yu Cao and Peng-wei Pan and Chao Wang},
title = {Identification of antiviral mimetic peptides with interferon α-2b-like activity from a random peptide library using a novel functional biopanning method},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {5},
year = {2016},
keywords = {},
abstract = {Aim: To screen for interferon (IFN) α-2b mimetic peptides with antiviral activity.
Methods: Selecting IFN receptor-binding peptides from a phage-display heptapeptide library using a novel functional biopanning method. This method was developed to identify peptides with activity against vesicular stomatitis virus (VSV) inducing cytopathic effects on WISH cells.
Results: Sixteen positive clones were obtained after 3 rounds of functional selection. Ten clones were picked from these positive clones according to the results of phage ELISA and were sequenced. The amino acid sequences homologous to IFNα-2b were defined by residues AB loop 31–37, BC loop 68–74, C helix 93–99, CD loop 106–112, D helix 115–121, DE loop 132–138, and E helix 143–161. Two of the peptides, designated clones T3 and T9, aligned with the IFNAR2-binding domains (AB loop and E helix), were synthesized and designated as IR-7 and KP-7, respectively. Both KP-7 and IR-7 were found to compete with GFP/IFNα-2b for receptor binding and mimicked the antiviral activity of IFNα-2b cooperatively.
Conclusion: Two IFNα-2b mimetic peptides with antiviral activity were derived from a phage-display heptapeptide library using a novel functional selection method.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4900}
}