@article{APS4852,
author = {Jian-ya Zhou and Xue-fen Wang and Fa-di Tang and Jian-ying Zhou and Guo-hua Lu and Yan Wang and Ru-lian Bian},
title = {Inhibitory effect of 1,8-cineol (eucalyptol) on Egr-1 expression in lipopolysaccharide-stimulated THP-1 cells},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {6},
year = {2016},
keywords = {},
abstract = {Aim: To study the effects of 1,8-cineol (eucalyptol) on the expression of early growth response factor-1 (Egr-1) and NF-κB in the human monocyte THP-1 cell line stimulated by lipopolysaccharide (LPS).
Methods: The THP-1 cells were incubated with serial doses of 1,8-cineol (1, 10, and 100 mg/L, 30 min) before being stimulated with LPS (1 mg/L, 30 min). The localization of Egr-1 in the THP-1 cells was detected by immunofluorescence and a laser scanning confocal microscope. The expression of Egr-1 in the nuclei and whole cell, and NF-κB in the nuclei, were measured by Western blot analysis.
Results: When stimulated by LPS, the FITC-labeled Egr-1 was detected mainly in the nuclei. Moreover, the expression of Egr-1 in the whole cell increased markedly compared with the control cells. 1,8-Cineol pretreatment decreased the expression of Egr-1 in both the nuclei and whole cell of the LPS-stimulated THP-1 cells, and this effect was concentration-dependent, but there was no reaction on the expression of NF-κB in the nuclei protein in the LPS-stimulated THP-1 cells.
Conclusion: In a concentration-dependent manner, 1,8-Cineol reduces LPS-induced Egr-1 expression in nuclei and in whole cell of THP-1 cells, but shows no effect on NF-κB expression.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4852}
}