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Effect of aclacinomycin B on DNA polymerase II of tumor cells

  
@article{APS4813,
	author = {Zhan-rong Li and Jian-bo Feng and Rui Han and Ming-biao Yin and Li-juan Xia},
	title = {Effect of aclacinomycin B on DNA polymerase II of tumor cells},
	journal = {Acta Pharmacologica Sinica},
	volume = {6},
	number = {3},
	year = {2016},
	keywords = {},
	abstract = {DEAE-cellulose, phosphocellulose and DNA-agarose affinity chromatography were used to isolate and purify the DNA polymerase II from L1210 cells and Lewis lung carcinoma. The enzyme showed a single polypeptide band on the profile of non-denaturing polyacrylamide electrophoresis and possessed high sensitively to N-ethylmaleimide(NEM).Aclacinomycin B inhibited potently DNA polymerase II from the 2 sources. The IC50 was 3.6 microg/ 100 microL and 5.3 microg/100 microL for Lewis lung carcinoma and the L 1210 enzymes, repectively.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/4813}
}