@article{APS4692,
author = {Ying Ji and Chen Liu and Yuan-ying Pei},
title = {Artificial pulmonary surfactant as a carrier for intratracheally instilled insulin},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {5},
year = {2016},
keywords = {},
abstract = {Aim: The relative bioavailabilities and effects on lung injury alleviation of 4 insulin-artificial pulmonary surfactant (INS-APS) preparations were studied in normal rats. The relationship between the minimal surface tension (γmin) of INS-APS and the absorption of insulin was also investigated.
Methods: Four formulations of APS [1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/lecithin/palmitic acid (PA), DPPC/1-hexadecanol (Hex)/tyloxapol (Tyl), DPPC/L-α-phosphatidyl-DL-glycerol sodium salt (PG), DPPC/Tyl] were prepared by thin-film sonication method and direct sonication. The γmin of 4 APS dispersions was examined with and without INS by pulsating with a bubble surface tensiometer. In vivo experiments were performed in which serum glucose change and the insulin level were measured by an enzymatic glucose reagent kit and a radioimmunology assay kit after IT to rats. The reduction in lung injury by INS-APS following 7 d of consecutive administration was evaluated by the pulmonary edema index (the weight ratio of wet lung to dry lung) and histopathology examination.
Results: The γmin of all APS dispersions were below 10 mN/m. There was no significant difference (P> 0.05) between the γmin of APS and the corresponding INS-APS. In vivo experiments showed a significant glucose level decrease and insulin absorption increase (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4692}
}