@article{APS4602,
author = {Hai-tao Zhang and Jun Wu and Hai-feng Zhang and Qi-feng Zhu},
title = {Efflux of potassium ion is an important reason of HL-60 cells apoptosis induced by tachyplesin},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {10},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the role of intercellular potassium in tachyplesin-induced HL-60 cells apoptosis.
Methods: The concentration of intercellular potassium, cell volume and mitochondrial membrane potential were examined by flow cytometry.
Results: The concentration of intercellular potassium reduced in a time-dependent manner in tachyplesin-treated HL-60 cells. In addition, the loss of mitochondrial membrane potential was tightly coupled with the shrinkage of cell volume. Different caspase inhibitors protected against DNA degradation but did not prevent the loss of HL-60 cell viability induced by tachyplesin. Ba2+, which was a kind of blocker of volume-regulatory K+ channels, increased the viability of tachyplesin-treated HL-60 cells and maintained mitochondrial membrane potential and cell volume.
Conclusion: Efflux of K+ was an important reason for apoptosis in tachyplesin-treated HL-60 cells. Efflux of K+ affected the viability of tachyplesin-treated HL-60 cells independent of the process of caspase activation.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4602}
}