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2-Amino-nonyl-6-methoxyl-tetralin muriate inhibits sterol C-14 reductase in the ergosterol biosynthetic pathway

   
@article{APS4478,
	author = {Rong-mei Liang and Yong-bing Cao and Kai-hua Fan and Yi Xu and Ping-hui Gao and You-jun Zhou and Bao-di Dai and Yong-hong Tan and Shi-hua Wang and Hui Tang and Hong-tao Liu and Yuan-ying Jiang},
	title = {2-Amino-nonyl-6-methoxyl-tetralin muriate inhibits sterol C-14 reductase in the ergosterol biosynthetic pathway},
	journal = {Acta Pharmacologica Sinica},
	volume = {30},
	number = {12},
	year = {2016},
	keywords = {},
	abstract = {Aim: To investigate the action mechanism of a novel chemical structural aminotetralin derivate, 2-Amino-Nonyl-6-Methoxyl-Tetralin Muriate (10b), against Candida albicans (C albicans) in the ergosterol biosynthetic pathway.
Methods: Antifungal susceptibility test of 10b was carried out using broth microdilution method, the action mechanism of 10b against C albicans was investigated by GC-MS spectrometry and real-time RT-PCR assay, and cytotoxicity of 10b in vitro was assessed by MTS/PMS reduction assay.
Results: 10b reduced the ergosterol content markedly, and the 50% ergosterol content inhibitory concentration (ECIC50 value) was 0.08 μg/mL. Although the sterol composition of 10b-grown cells was completely identical with that of erg24 strain, the content of ergosta-8,14,22-trienol in 10b-grown cells was much higher than that in erg24 strain. Real-time RT-PCR assay revealed a global upregulation of sterol metabolism genes. In addition, the 50% inhibitory concentration (IC50 value) of 10b was 11.30 μg/mL for murine embryonic fibroblasts and 35.70 μg/mL for human normal liver cells.
Conclusion: 10b possessed a mode of action different from that of azoles and morpholines, whose targets were sterol C-14 reductase (encoded by ERG24 gene) and sterol C-5 desaturase (encoded by ERG3) related enzyme. Although 10b seemed to reduce MTS/PMS reduction in a dose dependent manner, IC50 value for mammalian cells was much higher than 50% minimum inhibitory concentration (MIC50) value for C albicans. This indicates that the formulation is preliminarily safe and warrants further study for possible human applications.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/4478}
}