@article{APS4420,
author = {Xue-mei Zhou and Jing-bo Sun and Hui-ping Yuan and Dong-lai Wu and Xin-rong Zhou and Da-wei Sun and Hong-yi Li and Zheng-bo Shao and Zhi-ren Zhang},
title = {A rat model for studying neural stem cell transplantation},
journal = {Acta Pharmacologica Sinica},
volume = {30},
number = {11},
year = {2016},
keywords = {},
abstract = {Aim: The goal of this project was to develop a rat model for neural stem cell (NSC) transplantation studies in which NSCs were modified with brain-derived neurotrophic factor (BDNF) genes that may permit extensive and reliable analysis of the transplants.
Methods: NSCs were cultured and purified by limiting dilution assay in vitro and infected with recombinant retrovirus pLXSN-BDNF (BDNF-NSCs) and retrovirus pLXSN (p-NSCs). The expression of BDNF genes in transgenic and control NSC groups was measured by FQ-PCR and ELISA assays. NSCs were then transplanted into the subretinal space of normal rat retinas in four groups, which included NSCs alone, BDNF-NSCs, phosphate buffered saline (PBS) control, and normal control. Survival, migration, and differentiation of donor cells in host retinas were observed with optical coherence tomography (OCT), Heidelberg retina angiograph (HRA), and immunohistochemistry, respectively.
Results: The results obtained by FQ-PCR demonstrated that the copy numbers of BDNF gene templates from BDNF-NSCs were the highest among the four groups (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4420}
}