@article{APS4391,
author = {Ru-wei Jiang and Xiao-guang Du and Xuan Zhang and Xin Wang and Ding-yu Hu and Tao Meng and Yue-lei Chen and Mei-yu Geng and Jing-kang Shen},
title = {Synthesis and bioassay of β-(1,4)-D-mannans as potential agents against Alzheimer's disease},
journal = {Acta Pharmacologica Sinica},
volume = {34},
number = {12},
year = {2016},
keywords = {},
abstract = {Aim: Oligomannurarate 971 derived from a marine plant has shown neuroprotective effects. In this study we synthesized a series of truncated derivatives of the oligosaccharide, and investigated the effect of these derivatives against Aβ peptide toxicity in vitro.
Methods: The sulfoxide method was applied to synthesize the derivatives. SH-SY5Y human neuroblastoma cells were treated with Aβ1-40 (2 μmol/L), and the cell viability was detected using a CCK8 assay.
Results: A series of β-(1,4)-D-mannosyl oligosaccharide, ranging from the disaccharide to the hexasaccharide, were synthesized. Addition of 10 μmol/L β-(1,4)-D-mannobiose 6, β-(1,4)-D-mannotriose 9 or β-(1,4)-D-mannotetraose 12 in SH-SY5Y cells significantly attenuated Aβ1-40-induced toxicity. The efficacies were similar to those caused by 10 μmol/L oligomannurarate 971 or alzhemed. Other oligosaccharides including oligomaltoses and oligocelluloses were less active.
Conclusion: Synthetic homogeneous short chain β-(1,4)-D-mannans shows neuroprotective effect against Aβ peptide toxicity similar to that of heterogeneous oligomannurarate 971 and alzhemed.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4391}
}