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Pharmacokinetics and tissue distribution of a novel PDE5 inhibitor, SK-3530, in rats

  
@article{APS4358,
	author = {Hye-hyun Yoo and Nam-sun Kim and Guang-jin Im and Dong-hyun Kim},
	title = {Pharmacokinetics and tissue distribution of a novel PDE5 inhibitor, SK-3530, in rats},
	journal = {Acta Pharmacologica Sinica},
	volume = {28},
	number = {8},
	year = {2016},
	keywords = {},
	abstract = {Aim: To investigate the pharmacokinetic profile and tissue distribution of a novel phosphodiesterase type 5 inhibitor, 5-ethyl-2-\{5-[4-(2-hydroxy-ethyl)-piperazine-1-sulfonyl]-2-propoxy-phenyl\}-7-propyl-3,5-dihydro-pyrrolo(3,2-d)pyrimidin-4-one (SK-3530), in rats after administration of the 14C-labeled compound.
Methods: The pharmacokinetic parameters of SK-3530 were measured based on the total radioactivity and parent SK-3530 concentration in rat plasma after intravenous and oral administration. The tissue distribution of total radioactivity after a single oral administration of [14C]SK-3530 at a dose of 40 mg/kg was assayed. The plasma protein binding rates of SK-3530 were assessed by in vitro and ex vivo assay.
Results: The total radioactivity profiles showed linear pharmacokinetics. The maximum plasma concentration and area under the curve of the parent SK3530 were 10%-20% compared to those of the total radioactivity. After the oral administration of [14C]SK-3530, the radioactivity was widely distributed in all tissues, and the tissue/plasma ratio of the radioactivity 1 h after administration was calculated as 0.5-2.6 with the exception of excretory organs. Arelatively high penetration was shown in the adrenal glands, liver, and lung. In vitro and ex vivo plasma protein binding assay by ultrafiltration showed a considerably high binding rate of more than 97%.
Conclusion: SK-3530 was relatively well absorbed in the gastrointestinal tract and showed linear pharmacokinetics over the investigated dose range. SK-3530 had low oral bioavailability due to a high, first-pass metabolism.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/4358}
}