@article{APS4354,
author = {Te-jen Lai and Sheng-feng Hsu and Te-mao Li and Horng-chaung Hsu and Jaung-geng Lin and Chin-jung Hsu and Ming-chih Chou and Meng-chih Lee and Shun-fa Yang and Yi-chin Fong},
title = {Alendronate inhibits cell invasion and MMP-2 secretion in human chondrosarcoma cell line},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {8},
year = {2016},
keywords = {},
abstract = {Aim: Chondrosarcoma is a malignant primary bone tumor that responds poorly to both chemotherapy and radiation therapy. The aim of the present study was to investigate the effect of alendronate, a bisphosphonate, on the invasion and migration of human chondrosarcoma cells (JJ012).
Methods: JJ012 cells were treated with alendronate of various concentrations up to 100 mumol/L for a specified period, and then gelatin zymography and matrigel invasion assay was performed to study the effects of alendronate on matrix metalloproteinase (MMP)-2 activity and the invasion ability of JJ012 cells, respectively.
Results: Our data showed that alendronate exerted a dose- and time-dependent inhibitory effect on the invasion and migration of JJ012 cells. Furthermore, gelatin zymography and RT-PCR showed that alendronate treatment decreased the activity and mRNA levels of MMP-2 in a concentration-dependent manner.
Conclusion: Our findings suggest that alendronate may reduce MMP-2 secretion at the transcriptional and translational levels, and inhibit the invasion of chondrosarcoma cell. Therefore, alendronate may be a potential candidate for the systemic therapy of chondro-sarcomas, as well as other malignant diseases.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4354}
}