@article{APS4347,
author = {Yun Liu and Yan Sun and Ting Zhu and Yu Xie and Jing Yu and Wen-lan Sun and Guo-xian Ding and Gang Hu},
title = {11 beta-hydroxysteroid dehydrogenase type 1 promotes differentiation of 3T3-L1 preadipocyte},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {8},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the relationship between 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1), a potential link between obesity and type 2 diabetes, and preadipocyte differentiation.
Methods: Mouse 11 beta-HSD1 siRNA plasmids were transfected into 3T3-L1 preadipocytes (a cell line derived from mouse Swiss3T3 cells that were isolated from mouse embryo), for examination of the effect of targeted 11 beta-HSD1 inhibition on differentiation of 3T3-L1 cells. Differentiation was stimulated with 3-isobutyl-1-methyxanthine, insulin, and dexamethasone. The transcription level of the genes was detected by real-time PCR.
Results: Lipid accumulation was significantly inhibited in cells transfected with mouse 11 beta-HSD1 siRNA compared with non-transfected 3T3-L1 cells. Fewer lipid droplets were detected in the transfected cells both prior to stimulation and after stimulation with differentiation-inducing reagents. The expression of adipocyte differentiation-associated markers such as lipoprotein lipase and fatty acid synthetase were downregulated in the transfected cells. Similarly, the expression of preadipocyte factor-1, an inhibitor of adipocyte differentiation, was downregulated upon stimulation of differentiation and had no changes in the transfected cells.
Conclusion: 11 beta-HSD1 can promote preadipocyte differentiation. Based on this, we propose that 11 beta-HSD1 may be an important candidate mediator of obesity and obesity-induced insulin resistance.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4347}
}