@article{APS4346,
author = {Xiao-fei An and Jiang-yi Yu and Bo-ying Chen and Su-lin Zhang},
title = {Role of hypothalamus nociceptin/orphanin FQ in pre-ovulatory luteinizing hormone surge of estrogen and progesterone-primed, ovariectomized rats},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {8},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the role of hypothalamus nociceptin/orphanin FQ (OFQ) and its endogenous receptor, the opioid receptor-like 1 receptor (ORL1 receptor) in the estrus cycle of female rats.
Method: Radioimmunoassay was used to detect the effect of the intracerebroventricular (icv) administration of OFQ and/or the ORL1 receptor antagonist [Nphe1]Nociceptin(1-13)NH2, that is, NC13 on luteinizing hormone (LH) levels of estrogen- and progesterone (EBP)-primed, ovariectomized (OVX) rats (EBP-primed OVX rats). RT-PCR, Western blotting, and immunohistochemistry techniques were adopted to observe the changes of OFQ and the ORL1 receptor in the pre-optic area (POA) and the medial basal hypothalamus (MBH) of the estrus cycle of female rat.
Results: Pre-ovulatory LH surges in EBP-primed, OVX rats were significantly reduced by icv administration of 20 and 200 nmol OFQ (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4346}
}