@article{APS4148,
author = {Yan Zhu and Shu-wu Xie and Jian-feng Zhang and Ting-ting Zhang and Jie-yun Zhou and Yang Cao and Lin Cao},
title = {Involvement of Bcl-2, Src, and ERα in gossypol-mediated growth inhibition and apoptosis in human uterine leiomyoma and myometrial cells},
journal = {Acta Pharmacologica Sinica},
volume = {31},
number = {12},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the effect of gossypol on the growth of cultured human uterine leiomyoma and myometrial cells, the level of Bcl-2 and the activity of Src and estrogen receptor (ERα).
Methods: Human uterine leiomyoma and adjacent normal myometrial cells were cultured in vitro. Both cell types were treated with a graded concentration of gossypol. Cell viability was assayed using CCK-8. Morphological change was observed with optical and electronic microscopy. Apoptosis was evaluated using TUNEL assay. Levels of Bcl-2, ERα and Src were analyzed using Western blotting.
Results: Gossypol significantly inhibited growth and promoted apoptosis in cultured human uterine leiomyoma cells with the IC50 value and its corresponding 95% confidence intervals (CI) of 6.5 (4.0–10.5), 9.0 (4.9–16.5), and 7.5 (4.0–14.1) μmol/L at 20, 40, and 60 h, respectively. Gossypol exerted inhibitory effects on the myometrial cells with the IC50 value and its 95% CI of 49.1 (28.3–85.0), 14.5 (7.7–27.4), and 2.6 (1.2–5.6) μmol/L at 20, 40, and 60 h, respectively. Compared with control, gossypol 0.1-3.0 μmol/L markedly decreased the protein expression of Bcl-2 (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4148}
}