@article{APS4019,
author = {Jie Yu and Hai-feng Zhang and Feng Wu and Qiu-xia Li and Heng Ma and Wen-yi Guo and Hai-chang Wang and Feng Gao},
title = {Insulin improves cardiomyocyte contractile function through enhancement of SERCA2a activity in simulated ischemia/reperfusion},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {7},
year = {2016},
keywords = {},
abstract = {Aim: Insulin exerts anti-apoptotic effects in both cardiomyocytes and coronary endothelial cells following ischemia/reperfusion (I/R) via the Akt-endothelial nitric oxide synthase survival signal pathway. This important insulin signaling might further contribute to the improvement of cardiac function after reperfusion. In this study, we tested the hypothesis that sarcoplasmic reticulum calcium-AT-Pase (SERCA2a) is involved in the insulin-induced improvement of cardiac contractile function following I/R.
Methods: Ventricular myocytes were enzymatically isolated from adult SD rats. Simulated I/R was induced by perfusing cells with chemical anoxic solution for 15 min followed by reperfusion with Tyrode's solution with or without insulin for 30 min. Myocyte shortening and intracellular calcium transients were assessed and underlying mechanisms were investigated.
Results: Reperfusion with insulin (10-7 mol/L) significantly improved the recovery of contractile function (n=15-20 myocytes from 6-8 hearts, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4019}
}