@article{APS4010,
author = {Li-jun Yao and Gang Wang and Kun-fu Ou-yang and Chao-liang Wei and Xian-hua Wang and Shi-rong Wang and Wei Yao and Hong-ping Huang and Jian-hong Luo and Cai-hong Wu and Jie Liu and Zhuan Zhou and He-ping Cheng},
title = {Ca2+ sparks and Ca2+ glows in superior cervical ganglion neurons},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {7},
year = {2016},
keywords = {},
abstract = {Aim: Ca2+ release from the endoplasmic reticulum (ER) is an integral component of neuronal Ca2+ signaling. The present study is to investigate properties of local Ca2+ release events in superior cervical ganglion (SCG) neurons.
Methods: Primary cultured SCG neurons were prepared from neonatal rats (P3-P7). Low concentration of caffeine was used to induce Ca2+ release from the ER Ca2+ store, and intracellular Ca2+was recorded by high-resolution line scan confocal imaging and the Ca2+ indicator Fluo-4.
Results: Two populations of local Ca2+ release events with distinct temporal characteristics were evoked by 1.5 mmol/L caffeine near the surface membrane in the soma and the neurites of SCG neurons. Brief events similar to classic Ca2+ sparks lasted a few hundreds of milliseconds, whereas long-lasting events displayed duration up to tens of seconds. Typical somatic and neurite sparks were of 0.3- and 0.52-fold increase in local Fluo-4 fluorescence, respectively. Typical Ca2+ glows were brighter (ΔF/F0 approximately 0.6), but were highly confined in space. The half maximum of full duration of neurite sparks was much longer than those in the soma (685 vs 381 ms).
Conclusion: Co-existence of Ca2+ sparks and Ca2+ glows in SCG neurons indicates distinctive local regulation of Ca2+ release kinetics. The local Ca2+ signals of variable, site-specific temporal length may bear important implications in encoding a \"memory\" of the trigger signal.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4010}
}