@article{APS3974,
author = {Shun-jiang Xu and Wei-juan Gao and Bin Cong and Chun-ling Ma and Shu-jin Li and Yi-ling Ling and Zhen-yong Gu and Yu-xia Yao},
title = {Effect of cholecystokinin octapeptide on diacylglycerol-PKC signaling pathway in rat pulmonary interstitial macrophages stimulated by lipopolysaccharide},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {12},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the effect of cholecystokinin octapeptide (CCK-8) on the diacylglycerol-protein kinase C (DAG-PKC) signaling pathway in rat pulmonary interstitial macrophages (PIM) stimulated by lipopolysaccaride (LPS).
Methods: The PIM from rat lung tissues were isolated using the collagenase digestion method combined with alveolar lavage and pulmonary vessel perfusion. DAG content and PKC activity were measured by radioenzymatic assay. The translocation of PKCzeta was determined by semi-quantitative immunoblot analysis.
Results: CCK-8, at high concentrations (1times10-6-1times10-5 mol/L), decreased DAG content and inhibited PKC activity and PKCzeta translocation compared with that in rat resting PIM of a control group (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3974}
}