@article{APS3943,
author = {Young-jin Im and Yun-kyung Lee and Hae-young Chung and Dong-soon Im},
title = {Multiple actions of lysophosphatidylcholine in human Jurkat T cells},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {6},
year = {2016},
keywords = {},
abstract = {Aim: To obtain pathophysiological meanings of lysophosphatidylcholine (LPC) through the investigation of the effects of LPC in Jurkat T cells.
Methods: We measured ROS generation, [Ca2+]i, and mitochondrial membrane potential (MMP) by fluorescent spectrometry in Jurkat T cells.
Results: We observed that LPC significantly increased the reactive oxygen species (ROS) level in human Jurkat T cells. Among structurally-related lysolipids and eleven synthetic LPCs with different acyl chain lengths, palmitoyl LPC increased ROS to the highest level. α-Tocopherol, an antioxidant, and rottlerin PKCδ inhibitor were inhibitory effects on LPC-induced ROS generation. LPC rapidly depolarized MMP and markedly elevated [Ca2+]i by Ca2+ influx across the plasma membrane. However, LPC-induced ROS increase seemed to not be related with LPC-induced depolarization of MMP or [Ca2+]i increase. G2A family G protein-coupled receptors (GPCR) for lysolipids were expressed in Jurkat T cells, however, evidence indicated that GPCR was not involved in LPC actions.
Conclusion: LPC induced several cellular changes in Jurkat T cells, including an increase of ROS generation in a PKCδ-dependent and GPCR-independent manner, increase of [Ca2+]i through Ca2+ influx, and decrease of MMP. LPC-induced actions in Jurkat T cells represent novel action modes of LPC that do not involve GPCR and multiple independent changes of intracellular signaling molecules.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3943}
}