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Antitumor activity of a novel recombinant mutant human tumor necrosis factor-related apoptosis-inducing ligand

   
@article{APS3935,
	author = {Fang Fang and Ai-ping Wang and Shi-fang Yang},
	title = {Antitumor activity of a novel recombinant mutant human tumor necrosis factor-related apoptosis-inducing ligand},
	journal = {Acta Pharmacologica Sinica},
	volume = {26},
	number = {11},
	year = {2016},
	keywords = {},
	abstract = {Aim: To investigate the antitumor activity and safety of a novel recombinant mutant human tumor necrosis factor-related apoptosis-inducing ligand (rmh TRAIL).
Methods: Antitumor activity of rmh TRAIL was evaluated by using several tumor cell lines by MTT assay in vitro, and by using a mouse xenograft model in vivo. rmh TRAIL-induced apoptosis in tumor cells was detected by cell death enzyme-linked immunosorbent assay (ELISA), TdT-mediated dUTP nick-end labeling (TUNEL) assay and flow cytometry. The safety of rmh TRAIL was also evaluated in several normal human cell lines.
Results: At the concentration of 0.32–1000 ng/mL, rmh TRAIL remarkably inhibited the proliferation of 5 tumor cell lines from lung, colon, and breast cancer compared with wild type (wt TRAIL) in vitro, whereas at the concentration of 1 ng/mL-10 mug/mL, rmh TRAIL showed no or mild cytotoxicity in the normal cell lines. rmh TRAIL (3,15 mg/kg, ip, once daily for 10 d) exerted a significant inhibition on the growth of xenograft tumor NCI-H460 in nude mice compared with the saline group (P},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/3935}
}