@article{APS3928,
author = {Xian-fang Meng and Xiao-jing Zou and Bin Peng and Jing Shi and Xin-min Guan and Chun Zhang},
title = {Inhibition of ethanol-induced toxicity by tanshinone IIA in PC12 cells},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {6},
year = {2016},
keywords = {},
abstract = {Aim: To observe the effects of tanshinone IIA (Tan IIA) on the neurotoxicity induced by ethanol in PC12 cells and to explore its protective role.
Methods: PC12 cell survival was measured by MTT assay. The formation of reactive oxygen species (ROS) and lactate dehydrogenase (LDH) release were detected by 2',7'-dichlorofluorescin (DCF) fluorescence and calorimetric method, respectively. The percentage of cell apoptosis was monitored by flow cytometry. The expression of p53 was detected by immuno-fluorescence and flow cytometry.
Results: Ethanol significantly impaired the survival of PC 12 cells as demonstrated by MTT assay. Ethanol also induced significant ROS formation and increased LDH release. Pre-incubation with Tan IIA in the culture medium significantly reversed these changes. Ethanol caused cell apoptosis and the upregulation of p53 protein. The anti-apoptosis effects of Tan IIA on ethanol-induced toxicity were accompanied by the downregulation of pro-apoptotic p53 protein expression.
Conclusion: Tan IIA can protect neurons from apoptosis and might serve as a potential therapeutic drug for neurological disorders induced by ethanol.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3928}
}