@article{APS3923,
author = {Long-rui Pan and Qiang Tang and Qin Fu and Ben-rong Hu and Ji-zhou Xiang and Jia-qing Qian},
title = {Roles of nitric oxide in protective effect of berberine in ethanol-induced gastric ulcer mice},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {11},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the protective effects of berberine on ethanol-induced gastric ulcer in mice.
Methods: Gastric ulcers were induced by oral ingestion of ethanol. Nitric oxide (NO) content was measured, and mRNA expression of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).
Results: The ulcer index (UI) at 1 h, 2 h, 3 h and 6 h after oral administration of ethanol was 23.8plusminus1.4, 23.3plusminus2.2, 22.3plusminus1.2 and 20.8plusminus1.1, respectively. The UI in the berberine-treated groups (5 mg/kg and 50 mg/kg) was less than the control group. The content of NO in the control group was 73.3plusminus7.3 muL/L, 94.0plusminus9.2 muL/L, 109.6plusminus 6.4 muL/L and 138.2plusminus10.2 muL/L in gastric juice and 5.8plusminus1.1 mumol/g protein, 8.3plusminus 1.1 mumol/g protein, 9.8plusminus 1.1 mumol/g protein and 11.9plusminus 1.2 mumol/g protein in gastric tissue at 1 h, 2 h, 3 h and 6 h, respectively, after the oral administration of ethanol. The content of NO in the berberine-treated groups (5 mg/kg and 50 mg/kg) was higher than the control group at 1 h after the oral administration of ethanol (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3923}
}