@article{APS3878,
author = {Zhi-gang Zhuang and Fei Fei and Ying Chen and Wei Jin},
title = {Suberoyl bis-hydroxamic acid induces p53-dependent apoptosis of MCF-7 breast cancer cells},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {12},
year = {2016},
keywords = {},
abstract = {Aim: To study the effects of suberoyl bis-hydroxamic acid (SBHA), an inhibitor of histone deacetylases, on the apoptosis of MCF-7 breast cancer cells.
Methods: Apoptosis in MCF-7 cells induced by SBHA was demonstrated by flow cytometric analysis, morphological observation, and DNA ladder. Mitochondrial membrane potential (ΔΨm) was measured using the fluorescent probe JC-1. The expressions of p53, p21, Bax, and PUMA were determined using RT-PCR or Western blotting analysis after the MCF-7 cells were treated with SBHA or p53 siRNA.
Results: SBHA induced apoptosis in MCF-7 cells. The expressions of p53, p21, Bax, and PUMA were induced, and ΔΨm collapsed after treatment with SBHA. p53 siRNA abrogated the SBHA-induced apoptosis and the expressions of p53, p21, Bax, and PUMA.
Conclusion: The activation of the p53 pathway is involved in SBHA-induced apoptosis in MCF-7 cells.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3878}
}