@article{APS3853,
author = {So-hyung Kim and Kyoung-ah Kang and Rui Zhang and Mei-jing Piao and Dong-ok Ko and Zhi-hong Wang and Sung-wook Chae and Sam-sik Kang and Keun-hwa Lee and Hee-kyoung Kang and Hyun-wook Kang and Jin-won Hyun},
title = {Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {11},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the anti-oxidant properties of esculetin (6,7-dihydro-xycoumarin) against H2O2-induced Chinese hamster lung fibroblast (V79-4) damage.
Methods: The radical scavenging activity was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and intracellular reactive oxygen species (ROS). In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a protein carbonyl ELISA kit. As well, cellular DNA damage was detected by Western blot and immuno-fluorescence image. Cell viability was assessed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results: Esculetin exhibited DPPH radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities. The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H2O2. Therefore, esculetin recovered cell viability exposed to H2O2.
Conclusion: Esculetin efficiently attenuated the oxidative stress induced cell damage via its anti-oxidant properties. As a result, esculetin may be useful in the development of functional food and raw materials of medicine.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3853}
}