@article{APS3842,
author = {Xiang-hui Li and Jin-chao Zhang and Sen-fang Sui and Meng-su Yang},
title = {Effect of daidzin, genistin, and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {9},
year = {2016},
keywords = {},
abstract = {Aim: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts.
Methods: MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed.
Results: Daidzin, genistin, and glycitin 1times10-8, 5times10-7, 1times10-6, 5times10-6, and 1times10-5 mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5times10-7 mol/L and genistin 1times10-6 mol/L promoted the osteogenesis of MSC. Genistin 1times10-8, 5times10-7, 1times10-6, 5times10-6, and 1times10-5 mol/L and glycitin 1times10-8, 1times10-6, and 1times10-5 mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1times10-8, 5times10-7, 1times10-6, 5times10-6, and 1times10-5 mol/L all inhibited the adipocytic transdifferentiation of osteoblasts.
Conclusions: Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3842}
}