@article{APS3832,
author = {Xiao-fei An and Hai-ping Chen and Shu-Ian Ma and Yi Feng and Jun-wei Hao and Bo-ying Chen},
title = {Involvement of nociceptin/orphanin FQ in release of hypothalamic GnRH mediated by ORL1 receptor in ovariectomized rats},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {9},
year = {2016},
keywords = {},
abstract = {Aim: To investigate effect of the nociceptin/orphanin FQ (OFQ) on hypothalamus gonadotropin-releasing hormone (GnRH) release in ovariectomized (OVX) rats.
Methods: GnRH radioimmunoassay (RIA) was used to study the effect of OFQ on GnRH release in hypothalamus slices in vitro. Push-pull perfusion and intracerebroventicular (icv) injection were used to examine the effect of OFQ on GnRH release in the hypothalamus medial preoptic area (POA) in vivo. Ovariectomies were performed on female Sprague-Dawley rats, and their plasma luteinizing hormone (LH) levels were measured after icv injection of OFQ with or without [Nphe1]NC(1-13)NH2,a competitive antagonist of opioid receptor-like 1 receptor (ORL1 receptor). Reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate the expression of the ORL1 receptor in rat pituitary.
Results: GnRH release from hypothalamus slices was inhibited 90 min after the administration of 2 mmol/L and 20 mmol/L OFQ (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3832}
}