@article{APS3810,
author = {Cheng-liang Zhang and Xiao-lei Zou and Jia-bei Peng and Ming Xiang},
title = {Immune tolerance induced by adoptive transfer of dendritic cells in an insulin-dependent diabetes mellitus murine model},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {1},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the effect and underlying mechanisms of immune-tolerance
induced by the adoptive transfer of bone marrow (BM)-derived dendritic cells
(DC) in insulin-dependent diabetes mellitus (IDDM) mice. Methods: The IDDM
model was established by a low dose of streptozotocin (STZ) in Balb/c mice. Two
DC subpopulations were generated from the BM cells with granulocyte-macrophage
colony-stimulating factor with or without interleukin-4. The purity and the T
cell stimulatory capability of DC were identified. These cells were used to modulate
autoimmune response in pre-diabetic mice. Blood glucose was examined
weekly; pancreas tissues were taken for histopathological analysis, and CD4+ T
cells were isolated to detect lymphocyte proliferation by MTT assay and the ratio
of CD4+CD25+ T cells by fluorescence-activated cell sorting (FACS). The cytokine
secretion was determined by ELISA analysis. Results: Two DC subsets were
generated from BM, which have phenotypes of mature DC (mDC) and immature
DC (iDC), respectively. The level of blood glucose decreased significantly by
transferring iDC (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3810}
}