@article{APS3809,
author = {Bin Zhou and Xiao-cang Cao and Zhi-hong Fang and Cui-lin Zheng and Zhi-bo Han and He Ren and Man-chiu Poon and Zhong-chao Han},
title = {Prevention of diabetic microangiopathy by prophylactic transplant of mobilized peripheral blood mononuclear cells},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {1},
year = {2016},
keywords = {},
abstract = {Aim: To investigate whether the prophylactic local delivery of mobilized peripheral
blood mononuclear cells (M-PBMNC) could prevent peripheral microangiopathy
in diabetic nude mice. Methods: Diabetic nude mice were induced with
intraperitoneal injections of streptozotocin. With the time course of diabetes, we
detected the capillary and arteriole density of mice adductor muscles by immunohistopathy.
In situ apoptosis was detected by using TdT-mediated dUTP nick
end labeling (TUNEL) methods. M-PBMNC were labeled and locally delivered to
the adductor muscles. Mononuclear cells were also isolated and cultured in vitro
for the detection and counting of endothelial progenitor cells(EPC). Results:
Rarefication of capillaries and arterioles, enhanced apoptosis in adductor muscles,
and reduced circulating EPC in diabetic nude mice. Prophylactic local delivery of
M-PBMNC halted the progression of microvascular rarefaction in hind-limb skeletal
muscles by inhibiting apoptosis. We detected the survival, migration and
incorporation of transplanted M-PBMNC into the murine vasculature in vivo. In
addition, more EPC were available from M-PBMNC than non-mobilized cells.
Conclusion: These results suggested that the prophylactic local delivery of
M-PBMNC may represent a novel approach for the treatment of microvascular
complications in diabetics.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3809}
}