@article{APS3770,
author = {Xiang-dong Zha and He-sheng Huang and Li-zhi Zhou and Jing Liu and Kang-sen Xu},
title = {Thrombin-like enzymes from venom gland of Deinagkistrodon acutus: cDNA cloning, mechanism of diversity and phylogenetic tree construction},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {2},
year = {2016},
keywords = {},
abstract = {Aim: To clone cDNAs of thrombin-like enzymes (TLEs) from venom gland of
Deinagkistrodon acutus and analyze the mechanisms by which their structural
diversity arose. Methods: Reverse transcription-polymerase chain reaction and
gene cloning techniques were used, and the cloned sequences were analyzed by
using bioinformatics tools. Results: Novel cDNAs of snake venom TLEs were
cloned. The possibilities of post-transcriptional recombination and horizontal
gene transfer are discussed. A phylogenetic tree was constructed. Conclusion:
The cDNAs of snake venom TLEs exhibit great diversification. There are several
types of structural variations. These variations may be attributable to certain
mechanisms including recombination.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3770}
}