@article{APS3762,
author = {Dan-yan Zhu and Yi-jia Lou},
title = {Icariin-mediated expression of cardiac genes and modulation of nitric oxide signaling pathway during differentiation of mouse embryonic stem cells into cardiomyocytes in vitro },
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To investigate effects of icariin on cardiac gene expression and the modulation of nitric oxide (NO) signal transduction during the differentiation of embryonic stem (ES) cells into cardiomyocytes in vitro.
Methods: The expression levels of cardiac developmental-dependent genes were measured using reverse transcription-polymerase chain reaction (RT-PCR). The chronotropic responses of cardiomyocytes to -adrenoceptor stimulation were determined. The levels of cAMP and cGMP in ES cells were measured using radioimmunoassay. Endogenous NO levels were measured by using the Griess reaction. Aminoguanidine (AG) was used to confirm the influence of icariin on the endogenous NO signal pathway.
Results: Icariin significantly elevated mRNA levels of cardiac transcription factors GATA4 and Nkx2.5 , and cardiac-specific - MHC , MLC-2v and - ARgenes in a concentration- and time-dependent manner (P },
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3762}
}