How to cite item

Inhibitory effect of Ca2+ on in vivo gene transfer by electroporation

  
@article{APS3761,
	author = {Yong-gang Zhao and Hui-li Lu and Jin-liang Peng and Yu-hong Xu},
	title = {Inhibitory effect of Ca2+ on in vivo gene transfer by electroporation},
	journal = {Acta Pharmacologica Sinica},
	volume = {27},
	number = {3},
	year = {2016},
	keywords = {},
	abstract = {Aim:  To investigate the specific effects of Ca2+on transgene expression during electroporation-mediated gene transfer in mice.
Methods:  Skeletal muscle and skin were subjected to in vivo electroporation with a luciferase reporter plasmid, with or without Ca2+and various other ions.
Results:  For in vivo electroporation, the presence of just 10 mmol/L Ca2+ in the DNA solution drastically reduced the resulting transgene expression, to less than 5% of control values. Only Ca2+, not other ions, caused inhibition, and the effect was not tissue specific. More surprisingly, even when Ca2+ ions were delivered by electroporation before or after DNA administration, similar effects were still observed.
Conclusion:  The inhibitory effect of Ca2+ on in vivo gene transfer by electroporation is specific, ie, the inhibitory effect may be related to the cell membrane properties after electroporation and the subsequent resealing event.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/3761}
}