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Action of aluminum on high voltage-dependent calcium current and its modulation by ginkgolide B

  
@article{APS3689,
	author = {Lei Chen and Chang-jin Liu and Ming Tang and Ai Li and Xin-wu Hu and Yi-mei Du and Jing-jing Shen and Yong-li Lu and Jurgen Heschler},
	title = {Action of aluminum on high voltage-dependent calcium current and its modulation by ginkgolide B},
	journal = {Acta Pharmacologica Sinica},
	volume = {26},
	number = {5},
	year = {2016},
	keywords = {},
	abstract = {Aim: To investigate the effect of aluminum (Al) on high voltage-dependent calcium current (IHVA) and its modulation by ginkgolide B (Gin B).
Methods: The whole-cell, patch-clamp technique was used to record IHVA from acutely isolated hippocampal CA1 pyramydal neurons in rats.
Results: Al 0.1 mmol/L (low concentration) reduced IHVA; Al 0.75 and 1.0 mmol/L (high concentrations) increased IHVA, and Al decreased and increased IHVA at intermediate concentrations of 0.25 and 0.5 mmol/L. The increase of IHVA by Al 1.0 mmol/L was enhanced by the adenylyl cyclase (AC) agonist forskolin and was partly abolished by the cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) antagonist H-89, whereas the decrease observed with Al 0.1 mmol/L was neither reversed by forskolin nor affected by H-89. Gin B had no effect on IHVA in normal neurons, but canceled the increase in IHVA by 1.0 mmol/L Al.
Conclusion: The results indicate that the mechanism of Al affecting IHVA differs at different concentrations, and this may be attributed to its complex actions. Gin B could prevent neurons from injury by inhibiting calcium influx.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/3689}
}