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Amino acid 1–209 is essential for PDX-1-mediated repression of human CMV IE promoter activity

   
@article{APS3637,
	author = {Jing Chen and Lei Chen and Ge Li and Lu Cheng and Yin Huang and Jia-xin Zhang and Wei-wei Fan and Da-ru Lu},
	title = {Amino acid 1–209 is essential for PDX-1-mediated repression of human CMV IE promoter activity},
	journal = {Acta Pharmacologica Sinica},
	volume = {27},
	number = {11},
	year = {2016},
	keywords = {},
	abstract = {Aim: To explore the different roles of pancreatic duodenal homeobox factors-1 (PDX-1) domains in PDX-1 mediated repression of human cytomegalovirus immediately early (CMV IE) promoter.
Methods: A series of truncated PDX-1 mutants were constructed. The binding of PDX-1 and CMV IE promoter was identified by electrophoretic mobility shift assay (EMSA). The dual-reporter assay was applied to examine the repression activities of PDX-1 mutants on CMV IE promoter. In addition, RNAi technology was used to specifically knock down the endogenous PDX-1 expression.
Results: The reporter assay indicated that compared to the mock controls (pEGFP-N2), overexpression of PDX-1 resulted in a 41% decrease of CMV IE promoter activity in the 293 cells (P},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/3637}
}