@article{APS3633,
author = {Jeong-yeh Yang and Jeung-hyun Koo and Young-gil Song and Kang-beom Kwon and Ju-hyung Lee and Hee-sook Sohn and Byung-hyun Park and Eun-chung Jhee and Jin-woo Park},
title = {Stimulation of melanogenesis by scoparone in B16 melanoma cells},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {11},
year = {2016},
keywords = {},
abstract = {Aim: The effect of coumarin derivatives on melanogenesis was investigated in B16 murine melanoma cells.
Methods: Melanin content and tyrosinase activity were analyzed spectrophotometrically. The expression of tyrosinase, tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2) were measured either by reverse transcription-polymerase chain reaction (RT-PCR) or Western blot.
Results: Among the coumarin derivatives studied, scoparone (6,7-dimethoxycoumarin) was the most potent; the 6- or 7-methoxy group was found to be essential for the stimulation of melanogenesis. The melanin content was greatly increased by scoparone in a dose-dependent manner; there was no cytotoxicity at the effective concentrations. Scoparone increased enzyme activity as well as protein and mRNA expression of tyrosinase. In addition, mRNA of TRP-1 and TRP-2 were also increased after treatment with scoparone. H-89, an inhibitor of protein kinase A (PKA), completely inhibited the scoparone-induced increase of melanogenesis and the tyrosinase protein.
Conclusion: These results suggest that scoparone-induced stimulation of melanogenesis is likely to occur at the transcriptional level of melanogenesis-related enzymes through PKA signaling.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3633}
}