@article{APS3597,
author = {Lian Li and Li-ke Zhang and Yong-zheng Pang and Chun-shui Pan and Yong-fen Qi and Li Chen and Xian Wang and Chao-shu Tang and Jing Zhang},
title = {Cardioprotective effects of ghrelin and des-octanoyl ghrelin on myocardial injury induced by isoproterenol in rats},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {5},
year = {2016},
keywords = {},
abstract = {Aim: To determine the cardioprotective action of ghrelin and des-octanoyl ghrelin in rats with isoproterenol-induced myocardial injury.
Methods: Rats were subcutaneously injected with isoproterenol (ISO; 20, 10, and 5 mg/kg) on d 1, 2 and 3, respectively, and then 3 mg/kg for the next 7 d with or without ghrelin or des-octanoyl-ghrelin (100 mug/kg, twice daily). Plasma ghrelin and growth hormone levels were assayed using radioimmunoassay methods. Growth hormone secretagogue receptor (GHSR) and ghrelin mRNA were determined using RT-PCR. The maximal binding capacity and the affinity for [3H] ghrelin were determined by receptor binding assays.
Results: Compared with controls, ISO-treated rats showed severe myocardial injury, cardiomegaly, infarction-like necrosis and massive fibrosis with increases in irradiated-ghrelin (ir-ghrelin) content in plasma by 67% and myocardia by 66% and in the mRNA level in the myocardia by 93% (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3597}
}