@article{APS3589,
author = {Xiao-lei SHI and Yu-dong QIU and Qiang LI and Ting XIE and Zhang-hua ZHU and Lei-lei CHEN and Lei LI and Yi-tao DING},
title = {Hepatocyte-like cells from directed differentiation of mouse bone marrow cells in vitro},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: To design the effective directed differentiation medium to differentiate bone
marrow cells into hepatocyte-like cells. Methods: Bone marrow cells were cultured
in the directed differentiation media including fibroblast growth factor-4
(FGF-4) and oncostatin M (OSM). Hepatocyte-like cells from directed differentiation
of bone marrow cells were identified through cell morphology, RNA expressions
by reverse transcriptase-polymerase chain reaction (RT-PCR), protein expressions
by Western blot, and hepatocellular synthesis and metabolism functions
by albumin ELISA, Periodic acid-Shiff staining and urea assay. Results:
Some epithelial-like cells or polygonal cells appeared and increased in the course
of the cell directed differentiation. Hepatocyte nucleur factor-3β (HNF-3β), albumin
(ALB), cytokeratin 18 (CK18), transthyretin (TTR), glucose-6-phosphate (G-
6-Pase), and tyrosine aminotransferase (TAT) mRNA were expressed in the course
of the directed differentiation. The directed differentiated cells on d 21 expressed
HNF-3β, ALB, and CK18 proteins. The directed differentiated cells produced
albumin and synthesized urea in a time-dependent manner. They could also synthesize
glycogen. Conclusion: Our differentiation media, including FGF-4 and
OSM, are effective to differentiate bone marrow cells into hepatocyte-like cells,
which could be used for hepatocyte resources for bioartificial liver or hepatocyte
transplantation.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3589}
}