@article{APS3575,
author = {Li GAO and Bao-en SHAN and Jing CHEN and Jiang-hui LIU and Da-xiang SONG and Bao-cheng ZHU},
title = {Effects of spider Macrothele raven venom on cell proliferation and cytotoxicity in HeLa cells},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim:To examine the effect of venom from the spider Macrothele raven on cell
proliferation and cytotoxicity in human cervical carcinoma, HeLa cells. Methods:
Morphological and biochemical signs of apoptosis appeared using acridine orange-
ethidium bromide (AO/EB) staining. Marked morphological changes in HeLa
cells after treatment with spider venom were observed using scanning electron
microscopy (SEM) and transmission electron microscopy (TEM). Cell proliferation
and cytotoxicity were determined by [methyl-3H] thymidine assay ([3H]TdR)
and lactate dehydrogenase (LDH) release, respectively. DNA fragmentation and
cell cycle distribution were monitored using flow cytometry. In addition, Western
blot analysis was used to evaluate the level of caspase-3 expression. In vivo
examination of the inhibition of the size of tumors in nude mice treated with spider
venom was measured. Results: Marked morphological changes were observed
using AO/EB staining, SEM and TEM assay. Spider venom at concentrations of
10–40 mg/L caused dose- and time-dependent inhibition of HeLa cell proliferation.
The ratio of apoptosis and necrosis increased. The activity of caspase-3 was
upregulated after spider venom treatment. In vivo study of tumor size revealed
that tumors significantly decreased in size from controls to tumors treated for 3
weeks with spider venom (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3575}
}