@article{APS3571,
author = {Yang-mei DENG and Qiang-min XIE and Shui-juan ZHANG and Ji-qiang CHEN and Qiu-huo YANG and Ru-lian BIAN},
title = {Changes of 5-lipoxygenase pathway and proinflammatory mediators in cerebral cortex and lung tissue of sensitized rats},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To explore the change of 5-lipoxygenase (5-LO) pathway expression and
proinflammatory mediators level of lung tissue and cerebral cortex, and the possible
regulatory mechanism through central nervous 5-LO pathways to pulmonary
inflammatory status in antigen repeated challenged rats. Methods: Four
groups of rats were treated as control, asthma model, asthma model treatment
with dexamethasone (DXM, 0.5 mg/kg, ip) and ketotifen (5 mg/kg, ig). Tumor
necrosis factor (TNF)-α, interleukin (IL)-4, interferon (IFN)-γ, and nitric oxide
(NO) were detected by ELISA kits. The mRNA expression of 5-LO and LTA4-
hydrolase (LTA4-H) was analyzed by reverse transcription-polymerase chain reaction
(RT-PCR), and the protein content of 5-LO was measured by Western blot.
Results: Increase of TNF-α, IL-4, NO level, and decrease of IFN-γ level in
bronchoalveolar lavage fluid (BALF) and cerebral cortex in sensitized rats were
shown after repeated antigen challenge. The expression of 5-LO and LTA4-H
mRNA, and 5-LO protein levels were increased in lung tissue and cerebral cortex
in asthma rats. In comparison with the asthma model, DXM significantly inhibited
the increase of cytokine levels and the expression of 5-LO pathway enzyme
(P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3571}
}