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RNA interference by expression of short hairpin RNAs suppresses bcl-xL gene expression in nasopharyngeal carcinoma cells

  
@article{APS3553,
	author = {Fang LIU and Cheng-wei HE and Yue-fei ZHANG and Ke-yuan ZHOU},
	title = {RNA interference by expression of short hairpin RNAs suppresses bcl-xL gene expression in nasopharyngeal carcinoma cells},
	journal = {Acta Pharmacologica Sinica},
	volume = {26},
	number = {2},
	year = {2016},
	keywords = {},
	abstract = {Aim:To evaluate a new plasmid mediated RNA interference (RNAi) system and
investigate whether knock-down of bcl-xL by short hairpin RNA (shRNA) can
induce apoptosis of human nasopharyngeal carcinoma (NPC) cell line CNE-2Z in
vitro.
Methods: The plasmid containing mU6 promoter was subcloned to yield the
pmU6 plasmid, recombinant plasmid expressing shRNA targeting bcl-xL gene was
designed and constructed, and were co-transfected cells with green fluorescence
protein expressing plasmid. Flow cytometry was used to evaluate transfection
efficiency, and RT-PCR and Western blot were applied to analyze bcl-xL mRNA
and protein levels, respectively.
Results:The shRNA expressed by the recombinant
plasmid efficiently suppressed bcl-xL gene expression and induced apoptosis
of NPC cells in vitro.
Conclusion: The recombinant plasmid can sufficiently
mediate RNAi in CNE-2Z cells, and knock-down of the bcl-xL expression by shRNA
significantly induced apoptosis in CNE-2Z cells. The results suggest this new
system, mediated RNAi can be used as a tool for the study of gene function and
gene therapy.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/3553}
}