@article{APS3549,
author = {Ming YANG and Hong-lin HUANG and Bing-yang ZHU and Qin-hui TUO and Duan-fang LIAO},
title = {Onychin inhibits proliferation of vascular smooth muscle cells by regulating cell cycle},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {2},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the effects of onychin on the proliferation of cultured rat
artery vascular smooth muscle cells (VSMCs) in the presence of 10% new-born
calf serum (NCS). Methods: Rat VSMCs were incubated with onychin 1–50
μmol/L or genistein 10 μmol/L in the presence of 10% NCS for 24 h. The proliferation
of VSMCs was measured by cell counting and MTS/PMS colorimetric
assays. Cell cycle progression was evaluated by flow cytometry. Retinoblastoma
(Rb) phosphorylation, and expression of cyclin D1 and cyclin E were measured by
Western blot assays. The tyrosine phosphorylation of ERK1/2 was examined by
immunoprecipitation techniques using anti-phospho-tyrosine antibodies. Results:
The proliferation of VSMCs was accelerated significantly in the presence of 10%
NCS. Onychin reduced the metabolic rate of MTS and the cell number of VSMCs
in the presence of 10% NCS in a dose-dependent manner. Flow cytometry analysis
revealed that the G1-phase fraction ratio in the onychin group was higher than
that in the 10% NCS group (85.2% vs 70.0%, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3549}
}