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Cardiac-specific deletion of BRG1 ameliorates ventricular arrhythmia in mice with myocardial infarction

   
@article{APS10984,
	author = {Jing Li and Zi-yue Ma and Yun-feng Cui and Ying-tao Cui and Xian-hui Dong and Yong-zhen Wang and Yu-yang Fu and Ya-dong Xue and Ting-ting Tong and Ying-zi Ding and Ya-mei Zhu and Hai-jun Huang and Ling Zhao and Hong-zhao Lv and Ling-zhao Xiong and Kai Zhang and Yu-xuan Han and Tao Ban and Rong Huo},
	title = {Cardiac-specific deletion of BRG1 ameliorates ventricular arrhythmia in mice with myocardial infarction},
	journal = {Acta Pharmacologica Sinica},
	volume = {45},
	number = {3},
	year = {2024},
	keywords = {},
	abstract = {Malignant ventricular arrhythmia (VA) after myocardial infarction (MI) is mainly caused by myocardial electrophysiological remodeling. Brahma-related gene 1 (BRG1) is an ATPase catalytic subunit that belongs to a family of chromatin remodeling complexes called Switch/Sucrose Non-Fermentable Chromatin (SWI/SNF). BRG1 has been reported as a molecular chaperone, interacting with various transcription factors or proteins to regulate transcription in cardiac diseases. In this study, we investigated the potential role of BRG1 in ion channel remodeling and VA after ischemic infarction. Myocardial infarction (MI) mice were established by ligating the left anterior descending (LAD) coronary artery, and electrocardiogram (ECG) was monitored. Epicardial conduction of MI mouse heart was characterized in Langendorff-perfused hearts using epicardial optical voltage mapping. Patch-clamping analysis was conducted in single ventricular cardiomyocytes isolated from the mice. We showed that BRG1 expression in the border zone was progressively increased in the first week following MI. Cardiac-specific deletion of BRG1 by tail vein injection of AAV9-BRG1-shRNA significantly ameliorated susceptibility to electrical-induced VA and shortened QTc intervals in MI mice. BRG1 knockdown significantly enhanced conduction velocity (CV) and reversed the prolonged action potential duration in MI mouse heart. Moreover, BRG1 knockdown improved the decreased densities of Na+ current (INa) and transient outward potassium current (Ito), as well as the expression of Nav1.5 and Kv4.3 in the border zone of MI mouse hearts and in hypoxia-treated neonatal mouse ventricular cardiomyocytes. We revealed that MI increased the binding among BRG1, T-cell factor 4 (TCF4) and β-catenin, forming a transcription complex, which suppressed the transcription activity of SCN5A and KCND3, thereby influencing the incidence of VA post-MI.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/10984}
}